2017-07-10 13:55:032025-05-24 00:26:46
description
transcriptional activator of the levD-levE-levF-levG-sacC operon
transcriptional activator of the [[gene|levD]]-[[gene|levE]]-[[gene|levF]]-[[gene|levG]]-[[gene|sacC ]]operon
locus
BSU27080
BSU_27080
proteinLength
938
935
geneLength
2814
2808
product
transcriptional activator, PRD-type(for SigL-dependent promoter)
transcriptional activator, PRD-type (for [[protein|SigL]]-dependent promoter)
ec
outlinks
bsu
BSU27080
BSU_27080
Gene
Coordinates
2,763,025 → 2,765,832
2,763,025 2,765,832
The protein
Catalyzed reaction/ biological activity
binding to the upstream activating sequence in front of the [[gene|levD]] gene results in activation of transcription of the ''[[gene|levD]]-[[gene|levE]]-[[gene|levF]]-[[gene|levG]]-[[gene|sacC]]'' operon, bining occurs in the presence of fructose
binding to the upstream activating sequence in front of the [[gene|levD]] gene results in activation of transcription of the [[gene|levD]]-[[gene|levE]]-[[gene|levF]]-[[gene|levG]]-[[gene|sacC]] operon, bining occurs in the presence of fructose
The protein
Protein family
[SW|Transcription factors activating transcription at SigL-dependent promoters]
[SW|Transcription factors activating transcription at SigL-dependent promoters]
[SW|PRD-containing transcription factors] (according to UniProt)
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|Sigma-54 factor interaction domain] (aa 117-348) (according to UniProt)
2 [SW|PRD domain]s (aa 468-573, aa 831-935) (according to UniProt)
[SW|PTS EIIA domain] type-4 (aa 574-711) (according to UniProt)
The protein
Modification
phosphorylation (His503, His582, His866) (according to Swiss-Prot)
The protein
additional information
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000052 PRODORIC2 database]
Biological materials
Mutant
BKE27080 ([[gene|levR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCTATCATCCTTTGTC, downstream forward: _UP4_TAAAAACAGTTTTTTCATAT
BKK27080 ([[gene|levR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTCTATCATCCTTTGTC, downstream forward: _UP4_TAAAAACAGTTTTTTCATAT